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Microbiology laboratory tests use techniques to spot microorganisms like bacteria, fungi, parasites, and viruses. Technologic changes have made significant progress in the various areas including bacteriology, mycology, mycobacteriology, parasitology, and virology during the last two decades in the field of diagnostic microbiology. although many infections can be diagnosed on the basis of clinical criteria alone, cultures and other laboratory tests allow infections to be diagnosed with much greater certainty, and certain infections such as bacteriuria, bacteremia, and fungal and viral infections cannot be diagnosed without cultures or other laboratory tests. Moreover, accurate antimicrobial susceptibility testing of clinical isolates is the only means of monitoring trends in antibiotic resistance.